{"id":1912,"date":"2025-12-25T19:53:47","date_gmt":"2025-12-25T18:53:47","guid":{"rendered":"https:\/\/www.laboratoireslelegard.com\/?post_type=product&p=1912"},"modified":"2025-12-25T19:57:51","modified_gmt":"2025-12-25T18:57:51","slug":"test-comet","status":"publish","type":"product","link":"https:\/\/www.laboratoireslelegard.com\/it_ch\/product-2\/test-comet\/","title":{"rendered":"Test COMET: single\u2011cell gel electrophoresis"},"content":{"rendered":"
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Test COMET:<\/b><\/p>

Le test COMET est une m\u00e9thode de r\u00e9f\u00e9rence pour \u00e9valuer la capacit\u00e9 d\u2019un ingr\u00e9dient ou d\u2019un produit cosm\u00e9tique \u00e0 provoquer des dommages \u00e0 l\u2019ADN, un indicateur essentiel du risque g\u00e9notoxique. Dans un environnement strictement contr\u00f4l\u00e9, des cellules cultiv\u00e9es en laboratoire sont expos\u00e9es au produit test\u00e9 selon des conditions reproduisant une exposition r\u00e9aliste.<\/span><\/span><\/span><\/span><\/p>\n

Le test COMET<\/span><\/span><\/span><\/span><\/strong> (ou <\/span><\/span><\/span><\/span>single\u2011cell gel electrophoresis<\/span><\/span><\/span><\/span><\/em>) est une m\u00e9thode de r\u00e9f\u00e9rence reconnue par l\u2019OCDE (TG\u202f489) pour mesurer les dommages \u00e0 l\u2019ADN induits par un ingr\u00e9dient ou un produit cosm\u00e9tique. Dans un environnement de laborat<\/span><\/span><\/span><\/span>oire strictement contr\u00f4l\u00e9, des cellules humaines ou animales cultiv\u00e9es sont expos\u00e9es \u00e0 la substance test\u00e9e \u00e0 des concentrations r\u00e9alistes. Si le compos\u00e9 provoque des cassures ou des modifications de l\u2019ADN, ces l\u00e9sions fragmentent le mat\u00e9riel g\u00e9n\u00e9tique.<\/p>\n

Lorsque l\u2019ADN subit un dommage, il se fragmente. Au cours de l\u2019\u00e9lectrophor\u00e8se, ces fragments migrent en dehors du noyau cellulaire et forment une structure caract\u00e9ristique appel\u00e9e \u00ab com\u00e8te \u00bb : la partie centrale repr\u00e9sente la t\u00eate (ADN intact), tandis que les fragments endommag\u00e9s forment la queue. La longueur, l\u2019intensit\u00e9 et l\u2019\u00e9tendue de cette queue sont mesur\u00e9es pr\u00e9cis\u00e9ment afin d\u2019\u00e9valuer le niveau de dommage subi par les cellules.<\/p>\n

Ce test, extr\u00eamement sensible, permet de d\u00e9tecter des alt\u00e9rations g\u00e9n\u00e9tiques tr\u00e8s faibles, parfois invisibles avec d\u2019autres m\u00e9thodes. Il constitue un outil indispensable pour valider la s\u00e9curit\u00e9 toxicologique des mati\u00e8res premi\u00e8res, des actifs naturels et des formulations innovantes destin\u00e9es aux march\u00e9s suisse et europ\u00e9en, o\u00f9 la g\u00e9notoxicit\u00e9 fait l\u2019objet d\u2019exigences r\u00e9glementaires strictes.<\/p>\n

Le test COMET constitue aujourd\u2019hui l\u2019outil le plus sensible et le plus accept\u00e9 pour d\u00e9montrer l\u2019absence de g\u00e9notoxicit\u00e9 d\u2019un ingr\u00e9dient ou d\u2019une formulation cosm\u00e9tique. En combinant sa haute r\u00e9solution analytique avec une conformit\u00e9 stricte aux exigences suisses, europ\u00e9ennes et britanniques, il permet aux fabricants de s\u00e9curiser leurs produits, d\u2019acc\u00e9l\u00e9rer l\u2019obtention du num\u00e9ro de notification et de gagner la confiance des consommateurs soucieux de la s\u00fbret\u00e9 g\u00e9n\u00e9tique de leurs soins.<\/p>\n

\n<\/p>

Principe de la technique<\/h3>\n
    \n
  1. \n

    Enrobage des cellules<\/strong> \u2013 Apr\u00e8s exposition, les cellules sont incorpor\u00e9es dans un gel d\u2019agarose.<\/p>\n<\/li>\n

  2. \n

    Lysat<\/strong> \u2013 Une solution alcaline d\u00e9nature les membranes et lib\u00e8re l\u2019ADN.<\/p>\n<\/li>\n

  3. \n

    \u00c9lectrophor\u00e8se<\/strong> \u2013 Sous l\u2019action d\u2019un champ \u00e9lectrique, les fragments d\u2019ADN migrent hors du noyau.<\/p>\n<\/li>\n

  4. \n

    Formation de la \u00ab\u202fcom\u00e8te\u202f\u00bb<\/strong> \u2013 L\u2019ADN intact forme la t\u00eate<\/em>, tandis que les fragments migrent et cr\u00e9ent une queue<\/em>.<\/p>\n<\/li>\n

  5. \n

    Analyse<\/strong> \u2013 La longueur, l\u2019intensit\u00e9 et le rapport t\u00eate\u2011queue sont quantifi\u00e9s \u00e0 l\u2019aide de logiciels d\u2019imagerie. Ces param\u00e8tres (taille de la queue, % d\u2019ADN dans la queue, indice de dommage) donnent une mesure pr\u00e9cise du potentiel g\u00e9notoxique<\/strong>.<\/p>\n<\/li>\n<\/ol>\n

    \n<\/h3>\n
      \n
    • \n

      Sensibilit\u00e9 exceptionnelle<\/strong>\u202f: il d\u00e9tecte des dommages g\u00e9n\u00e9tiques tr\u00e8s faibles, souvent invisibles avec d\u2019autres m\u00e9thodes (ex.\u202fmicronucleus).<\/p>\n<\/li>\n

    • \n

      Applicabilit\u00e9 large<\/strong>\u202f: convient aux mati\u00e8res premi\u00e8res, aux actifs naturels, aux extraits biotechnologiques et aux formulations finales.<\/p>\n<\/li>\n

    • \n

      Conformit\u00e9 r\u00e9glementaire<\/strong>\u202f: les autorit\u00e9s suisses (OCos\/OSAV) et europ\u00e9ennes (R\u00e8glement\u202fCE\u202f1223\/2009) exigent une \u00e9valuation de la g\u00e9notoxicit\u00e9 pour les ingr\u00e9dients destin\u00e9s \u00e0 un usage cutan\u00e9 prolong\u00e9 ou \u00e0 proximit\u00e9 des muqueuses.<\/p>\n<\/li>\n

    • \n

      Int\u00e9gration au DIP\/PIF<\/strong>\u202f: les r\u00e9sultats sont directement report\u00e9s dans le Dossier d\u2019Information Produit<\/strong> (DIP) ou le Product Information File<\/strong> (PIF), renfor\u00e7ant la cr\u00e9dibilit\u00e9 du dossier de mise sur le march\u00e9.<\/p>\n<\/li>\n<\/ul>\n

      <\/p>\n

      \n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

      Test COMET: Le test COMET est une m\u00e9thode de r\u00e9f\u00e9rence pour \u00e9valuer la capacit\u00e9 d\u2019un ingr\u00e9dient ou d\u2019un produit cosm\u00e9tique \u00e0 provoquer des dommages \u00e0 l\u2019ADN, un indicateur essentiel du risque g\u00e9notoxique. Dans un environnement strictement contr\u00f4l\u00e9, des cellules cultiv\u00e9es en laboratoire sont expos\u00e9es au produit test\u00e9 selon des conditions reproduisant une exposition r\u00e9aliste. Le […]<\/p>","protected":false},"featured_media":1735,"template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[151],"product_tag":[113,116,112,114,115,117],"class_list":{"0":"post-1912","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-analyse","7":"product_tag-activite","8":"product_tag-activity","9":"product_tag-analyse","10":"product_tag-eau","11":"product_tag-micro-organismes","12":"product_tag-water","14":"first","15":"instock","16":"shipping-taxable","17":"product-type-simple"},"acf":[],"yoast_head":"\nTest COMET: single\u2011cell gel electrophoresis<\/title>\n<meta name=\"description\" content=\"COMET test\u202f: analyse ultra\u2011sensible de l\u2019ADN, d\u00e9tecte la g\u00e9notoxicit\u00e9 et assure la conformit\u00e9 cosm\u00e9tique Suisse\u2011UE pour les ingr\u00e9dients innovants!!!\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" 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