{"id":1903,"date":"2025-12-25T19:34:46","date_gmt":"2025-12-25T18:34:46","guid":{"rendered":"https:\/\/www.laboratoireslelegard.com\/?post_type=product&p=1903"},"modified":"2025-12-25T19:48:48","modified_gmt":"2025-12-25T18:48:48","slug":"het-cam-irritation-occulaire","status":"publish","type":"product","link":"https:\/\/www.laboratoireslelegard.com\/it_ch\/product-2\/het-cam-irritation-occulaire\/","title":{"rendered":"HET\u2011CAM\u202f: test d\u2019\u00e9valuation de l\u2019irritation oculaire"},"content":{"rendered":"
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HET\u2011CAM\u202f: test alternatif non animal pour l\u2019\u00e9valuation de l\u2019irritation oculaire<\/strong><\/p><\/div>

Le HET\u2011CAM<\/strong> (Hen\u2019s Egg Test \u2013 Chorioallantoic Membrane) est aujourd\u2019hui la m\u00e9thode de r\u00e9f\u00e9rence lorsqu\u2019il s\u2019agit d\u2019\u00e9valuer le potentiel irritant d\u2019un produit cosm\u00e9tique sur les yeux sans recourir \u00e0 l\u2019exp\u00e9rimentation animale. Conform\u00e9ment aux exigences europ\u00e9ennes (R\u00e8glement\u202fCE\u202f1223\/2009) et suisses (OCos\/OSAV), ce test exploite la membrane chorioallanto\u00efdienne d\u2019un \u0153uf de poule incub\u00e9\u202f: une membrane ultra\u2011fine, tr\u00e8s vascularis\u00e9e et extr\u00eamement sensible aux agents chimiques irritants.<\/p><\/div>

Principe du test<\/h3><\/div>
  1. Incubation de l\u2019\u0153uf<\/strong> \u2013 L\u2019\u0153uf est incub\u00e9 pendant 9 \u00e0 10\u202fjours \u00e0 37\u202f\u00b0C, moment o\u00f9 la membrane chorioallanto\u00efdienne est pleinement d\u00e9velopp\u00e9e.<\/li>
  2. Application du produit<\/strong> \u2013 Une petite goutte (10\u202f\u00b5L) du produit cosm\u00e9tique \u00e0 tester est d\u00e9pos\u00e9e directement sur la membrane.<\/li>
  3. Marquage des l\u00e9sions<\/strong> \u2013 Des colorants sp\u00e9cifiques (g\u00e9n\u00e9ralement le rouge de ph\u00e9nol\u2011sulfonate) sont ajout\u00e9s pour mettre en \u00e9vidence les modifications vasculaires : rougeurs, micro\u2011coagulations, h\u00e9morragies ou rupture des vaisseaux.<\/li>
  4. Observation et scoring<\/strong> \u2013 La membrane est observ\u00e9e pendant 5\u202fminutes, puis \u00e0 30\u202fminutes. L\u2019\u00e9tendue et la rapidit\u00e9 des changements vasculaires sont not\u00e9es selon un tableau de scoring standardis\u00e9 (0\u202f=\u202faucune irritation, 5\u202f=\u202firritation s\u00e9v\u00e8re).<\/li><\/ol>

    Avantages du HET\u2011CAM<\/h3>
    • Absence d\u2019animal mature<\/strong>\u202f: la m\u00e9thode utilise un embryon, ce qui la classe parmi les techniques 3R (Replacement).<\/li>
    • Sensibilit\u00e9 \u00e9lev\u00e9e<\/strong>\u202f: m\u00eame les irritants l\u00e9gers provoquent des r\u00e9ponses vasculaires visibles, permettant une classification fine selon le syst\u00e8me GHS.<\/li>
    • Rapidement ex\u00e9cutable<\/strong>\u202f: le protocole complet se d\u00e9roule en moins d\u2019une heure, id\u00e9al pour les phases de d\u00e9veloppement o\u00f9 les d\u00e9lais sont critiques.<\/li>
    • Co\u00fbt r\u00e9duit<\/strong>\u202f: aucun besoin d\u2019\u00e9quipements co\u00fbteux ni de maintenance d\u2019animaux, ce qui rend le test \u00e9conomiquement attractif pour les laboratoires de R&D.<\/li><\/ul>

      Limites \u00e0 conna\u00eetre<\/h3>
      • Pas de mesure de perm\u00e9abilit\u00e9 corn\u00e9enne<\/strong>\u202f: contrairement au BCOP ou aux mod\u00e8les de corn\u00e9e reconstitu\u00e9e, le HET\u2011CAM ne fournit pas d\u2019information directe sur la p\u00e9n\u00e9tration du produit dans la corn\u00e9e.<\/li>
      • Interpr\u00e9tation semi\u2011quantitative<\/strong>\u202f: le scoring repose sur l\u2019observation visuelle, ce qui peut introduire une variabilit\u00e9 inter\u2011op\u00e9rateur si les crit\u00e8res ne sont pas strictement harmonis\u00e9s.<\/li>
      • Applicabilit\u00e9 aux formulations tr\u00e8s lipophiles<\/strong>\u202f: les huiles \u00e9paisses peuvent ne pas se r\u00e9partir uniform\u00e9ment sur la membrane, n\u00e9cessitant parfois l\u2019ajout d\u2019un solvant compatible.<\/li><\/ul>

        Int\u00e9gration dans le dossier r\u00e9glementaire<\/h3>

        Les r\u00e9sultats du HET\u2011CAM sont incorpor\u00e9s dans le DIP\/PIF<\/strong> (Dossier d\u2019Information Produit \/ Product Information File) et servent \u00e0 justifier la classification d\u2019irritation oculaire du produit. Ils sont particuli\u00e8rement pertinents pour les cr\u00e8mes contour des yeux, mascaras, d\u00e9maquillants oculaires<\/strong> et tout autre produit susceptible d\u2019entrer en contact avec la zone oculaire.<\/p>

        En combinant le HET\u2011CAM avec d\u2019autres tests in\u202fvitro (BCOP, RhCE) pour couvrir la perm\u00e9abilit\u00e9 corn\u00e9enne, les fabricants obtiennent un dossier complet, conforme aux exigences suisses, europ\u00e9ennes et britanniques. Cette approche holistique renforce la cr\u00e9dibilit\u00e9 scientifique du produit, acc\u00e9l\u00e8re l\u2019obtention du num\u00e9ro de notification et rassure les consommateurs quant \u00e0 la s\u00e9curit\u00e9 de leurs soins pr\u00e8s des yeux.<\/p><\/div>

        <\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

        HET\u2011CAM\u202f: test alternatif non animal pour l\u2019\u00e9valuation de l\u2019irritation oculaire Le HET\u2011CAM (Hen\u2019s Egg Test \u2013 Chorioallantoic Membrane) est aujourd\u2019hui la m\u00e9thode de r\u00e9f\u00e9rence lorsqu\u2019il s\u2019agit d\u2019\u00e9valuer le potentiel irritant d\u2019un produit cosm\u00e9tique sur les yeux sans recourir \u00e0 l\u2019exp\u00e9rimentation animale. Conform\u00e9ment aux exigences europ\u00e9ennes (R\u00e8glement\u202fCE\u202f1223\/2009) et suisses (OCos\/OSAV), ce test exploite la membrane chorioallanto\u00efdienne […]<\/p>","protected":false},"featured_media":1738,"template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[151],"product_tag":[113,116,112,114,115,117],"class_list":["post-1903","product","type-product","status-publish","has-post-thumbnail","product_cat-analyse","product_tag-activite","product_tag-activity","product_tag-analyse","product_tag-eau","product_tag-micro-organismes","product_tag-water","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\nHET\u2011CAM\u202f: test d\u2019\u00e9valuation de l\u2019irritation oculaire<\/title>\n<meta name=\"description\" content=\"HET-CAM : test alternatif sans animaux pour \u00e9valuer l\u2019irritation oculaire, fiable pour cosm\u00e9tiques, conforme aux exigences UE\u2011Suisse et la r\u00e9glementation!\" \/>\n<meta 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