{"id":1922,"date":"2025-12-25T20:00:54","date_gmt":"2025-12-25T19:00:54","guid":{"rendered":"https:\/\/www.laboratoireslelegard.com\/?post_type=product&p=1922"},"modified":"2025-12-25T20:08:47","modified_gmt":"2025-12-25T19:08:47","slug":"test-del-dna","status":"publish","type":"product","link":"https:\/\/www.laboratoireslelegard.com\/it\/product\/test-del-dna\/","title":{"rendered":"Test TUNEL: rilevare la frammentazione del DNA"},"content":{"rendered":"
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Test TUNEL: rilevamento ultrasensibile dell'apoptosi per garantire la sicurezza cellulare dei cosmetici<\/strong><\/p><\/div>

Il test TUNEL \u00e8 un metodo essenziale per rilevare la frammentazione del DNA, un marcatore caratteristico della morte cellulare programmata, o apoptosi. Consente di determinare se un ingrediente o un prodotto cosmetico pu\u00f2 indurre un'apoptosi eccessiva o indesiderata nelle cellule esposte, un parametro importante per garantire la sicurezza biologica delle formulazioni.<\/p>

Le cellule vengono prima messe a contatto con il prodotto in condizioni rigorosamente controllate, rappresentative del suo utilizzo reale. I frammenti di DNA generati durante il processo apoptotico vengono quindi marcati con indicatori fluorescenti o colorimetrici, che consentono di visualizzare con precisione le cellule colpite. La percentuale di cellule marcate e l'intensit\u00e0 del segnale consentono di valutare l'entit\u00e0 dell'effetto osservato.<\/p>

Un tasso elevato di cellule positive pu\u00f2 rivelare un potenziale rischio per la vitalit\u00e0 cellulare, mentre un tasso basso o nullo conferma la buona tolleranza del prodotto a questo livello biologico. Grazie alla sua elevata sensibilit\u00e0, il test TUNEL costituisce uno strumento complementare alle altre analisi tossicologiche e genetiche utilizzate in Svizzera e in Europa per convalidare l'innocuit\u00e0 dei principi attivi e delle formulazioni innovative.<\/p>

Il test TUNEL<\/strong> (Terminal deoxynucleotidyl transferase dUTP Nick End Labelling) \u00e8 oggi il metodo di riferimento per identificare la frammentazione del DNA, marcatore definitivo della morte cellulare programmata (apoptosi). Nel campo cosmetico, consente di verificare che un ingrediente o una formulazione non provochi un'apoptosi eccessiva, criterio essenziale per la sicurezza biologica dei prodotti destinati alla pelle o alle mucose.<\/p><\/div>

Principio del test<\/h3><\/div>
  1. Esposizione controllata<\/strong> \u2013 Le cellule umane (cheratinociti, fibroblasti o cellule congiuntivali a seconda del prodotto testato) vengono incubate con il prodotto a concentrazioni che riproducono le condizioni di utilizzo reali.<\/li>
  2. Marcatura delle rotture del DNA<\/strong> \u2013 La nucleotidil-transferasi terminale (TdT) aggiunge nucleotidi marcati (fluorocromi: FITC, Alexa 488; o substrati colorimetrici) alle estremit\u00e0 3'-OH dei frammenti di DNA generati durante l'apoptosi.<\/li>
  3. Rilevamento<\/strong> \u2013 Le cellule marcate vengono visualizzate al microscopio a fluorescenza o analizzate mediante citometria a flusso. La percentuale di cellule positive e l'intensit\u00e0 del segnale quantificano l'entit\u00e0 dell'apoptosi indotta.<\/li><\/ol>

    Perch\u00e9 il TUNEL?<\/h3>
    • Elevata sensibilit\u00e0<\/strong>\u202f: rileva anche bassi livelli di frammentazione del DNA, spesso invisibili con i test di vitalit\u00e0 convenzionali (MTT, Trypan Blue).<\/li><\/ul><\/div>
      • \u00a0<\/li>
      • Specificit\u00e0<\/strong>\u202f: la colorazione avviene solo quando i filamenti di DNA sono effettivamente tagliati, evitando falsi positivi legati a danni superficiali.<\/li>
      • Conformit\u00e0 ai requisiti normativi<\/strong>\u202f: le autorit\u00e0 svizzere (OCos\/OSAV) ed europee (Regolamento CE 1223\/2009) richiedono una valutazione completa della tossicit\u00e0 cellulare per i principi attivi, in particolare quelli di origine naturale o biotecnologica.<\/li>
      • Integrazione nel DIP\/PIF<\/strong>\u202f: i risultati (percentuale di cellule TUNEL-positive, curve dose-risposta) vengono riportati direttamente nel Fascicolo informativo sul prodotto (DIP) o nel Product Information File (PIF), rafforzando il fascicolo di immissione in commercio.<\/li><\/ul>
        <\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

        Test TUNEL\u202f: d\u00e9tection ultrasensible de l\u2019apoptose pour garantir la s\u00e9curit\u00e9 cellulaire des cosm\u00e9tiques Le test TUNEL est une m\u00e9thode essentielle pour d\u00e9tecter la fragmentation de l\u2019ADN, un marqueur caract\u00e9ristique de la mort cellulaire programm\u00e9e, ou apoptose. Il permet de d\u00e9terminer si un ingr\u00e9dient ou un produit cosm\u00e9tique peut induire une apoptose excessive ou non d\u00e9sir\u00e9e […]<\/p>","protected":false},"featured_media":1736,"template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[151],"product_tag":[113,116,112,114,115,117],"class_list":["post-1922","product","type-product","status-publish","has-post-thumbnail","product_cat-analyse","product_tag-activite","product_tag-activity","product_tag-analyse","product_tag-eau","product_tag-micro-organismes","product_tag-water","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\nTest TUNEL: d\u00e9tecter la fragmentation de l\u2019ADN<\/title>\n<meta name=\"description\" content=\"Test TUNEL\u202f: d\u00e9tection ultrasensible de la fragmentation ADN et de l\u2019apoptose, assure la s\u00e9curit\u00e9 des cosm\u00e9tiques, conforme aux exigences Suisse\u2011UE.\" \/>\n<meta name=\"robots\" content=\"index, follow, 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