{"id":1912,"date":"2025-12-25T19:53:47","date_gmt":"2025-12-25T18:53:47","guid":{"rendered":"https:\/\/www.laboratoireslelegard.com\/?post_type=product&p=1912"},"modified":"2025-12-25T19:57:51","modified_gmt":"2025-12-25T18:57:51","slug":"test-comet","status":"publish","type":"product","link":"https:\/\/www.laboratoireslelegard.com\/it\/product\/test-comet\/","title":{"rendered":"Test COMET: elettroforesi su gel a singola cellula"},"content":{"rendered":"
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Test COMET:<\/b><\/p>

Il test COMET \u00e8 un metodo di riferimento per valutare la capacit\u00e0 di un ingrediente o di un prodotto cosmetico di provocare danni al DNA, un indicatore essenziale del rischio genotossico. In un ambiente rigorosamente controllato, le cellule coltivate in laboratorio vengono esposte al prodotto. testato in condizioni che riproducono un'esposizione realistica.<\/span><\/span><\/span><\/span><\/p>\n

Il test COMET<\/span><\/span><\/span><\/span><\/strong> (o <\/span><\/span><\/span><\/span>elettroforesi su gel a singola cellula<\/span><\/span><\/span><\/span><\/em>) \u00e8 un metodo di riferimento riconosciuto dall'OCSE (TG 489) per misurare i danni al DNA indotti da un ingrediente o da un prodotto cosmetico. In un ambiente di laboratorio<\/span><\/span><\/span><\/span>In un ambiente rigorosamente controllato, cellule umane o animali coltivate vengono esposte alla sostanza testata a concentrazioni realistiche. Se il composto provoca rotture o alterazioni del DNA, tali lesioni frammentano il materiale genetico.<\/p>\n

Quando il DNA subisce un danno, si frammenta. Durante l'elettroforesi, questi frammenti migrano fuori dal nucleo cellulare e formano una struttura caratteristica chiamata \u00abcometa\u00bb: la parte centrale rappresenta la testa (DNA intatto), mentre i frammenti danneggiati formano la coda. La lunghezza, l'intensit\u00e0 e l'estensione di questa coda vengono misurate con precisione per valutare il livello di danno subito dalle cellule.<\/p>\n

Questo test, estremamente sensibile, consente di rilevare alterazioni genetiche molto lievi, talvolta invisibili con altri metodi. Costituisce uno strumento indispensabile per convalidare la sicurezza tossicologica delle materie prime, dei principi attivi naturali e delle formulazioni innovative destinate ai mercati svizzero ed europeo, dove la genotossicit\u00e0 \u00e8 soggetta a severi requisiti normativi.<\/p>\n

Il test COMET \u00e8 oggi lo strumento pi\u00f9 sensibile e pi\u00f9 accettato per dimostrare l'assenza di genotossicit\u00e0 di un ingrediente o di una formulazione cosmetica. Combinando la sua elevata risoluzione analitica con una rigorosa conformit\u00e0 ai requisiti svizzeri, europei e britannici, consente ai produttori di garantire la sicurezza dei loro prodotti, accelerare l'ottenimento del numero di notifica e conquistare la fiducia dei consumatori attenti alla sicurezza genetica dei loro prodotti di bellezza.<\/p>\n

\n<\/p>

Principio della tecnica<\/h3>\n
    \n
  1. \n

    Rivestimento delle celle<\/strong> \u2013 Dopo l'esposizione, le cellule vengono incorporate in un gel di agarosio.<\/p>\n<\/li>\n

  2. \n

    Lysato<\/strong> \u2013 Una soluzione alcalina denatura le membrane e libera il DNA.<\/p>\n<\/li>\n

  3. \n

    Elettroforesi<\/strong> \u2013 Sotto l'azione di un campo elettrico, i frammenti di DNA migrano fuori dal nucleo.<\/p>\n<\/li>\n

  4. \n

    Formazione della \u00abcometa\u00bb<\/strong> \u2013 Il DNA intatto forma la testa<\/em>, mentre i frammenti migrano e creano una coda<\/em>.<\/p>\n<\/li>\n

  5. \n

    Analisi<\/strong> \u2013 La lunghezza, l'intensit\u00e0 e il rapporto testa-coda vengono quantificati utilizzando software di imaging. Questi parametri (dimensione della coda, % di DNA nella coda, indice di danno) forniscono una misura precisa del potenziale genotossico<\/strong>.<\/p>\n<\/li>\n<\/ol>\n

    \n<\/h3>\n
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      Sensibilit\u00e0 eccezionale<\/strong>\u202f: rileva danni genetici molto lievi, spesso invisibili con altri metodi (ad es. micronuclei).<\/p>\n<\/li>\n

    • \n

      Ampia applicabilit\u00e0<\/strong>\u202f: adatto a materie prime, principi attivi naturali, estratti biotecnologici e formulazioni finali.<\/p>\n<\/li>\n

    • \n

      Conformit\u00e0 normativa<\/strong>\u202f: le autorit\u00e0 svizzere (OCos\/OSAV) ed europee (Regolamento CE 1223\/2009) richiedono una valutazione della genotossicit\u00e0 per gli ingredienti destinati a un uso prolungato sulla pelle o in prossimit\u00e0 delle mucose.<\/p>\n<\/li>\n

    • \n

      Integrazione nel DIP\/PIF<\/strong>\u202f: i risultati vengono riportati direttamente nel Scheda informativa sul prodotto<\/strong> (DIP) o il Scheda informativa sul prodotto<\/strong> (PIF), rafforzando la credibilit\u00e0 del dossier di immissione in commercio.<\/p>\n<\/li>\n<\/ul>\n

      <\/p>\n

      \n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

      Test COMET: Le test COMET est une m\u00e9thode de r\u00e9f\u00e9rence pour \u00e9valuer la capacit\u00e9 d\u2019un ingr\u00e9dient ou d\u2019un produit cosm\u00e9tique \u00e0 provoquer des dommages \u00e0 l\u2019ADN, un indicateur essentiel du risque g\u00e9notoxique. Dans un environnement strictement contr\u00f4l\u00e9, des cellules cultiv\u00e9es en laboratoire sont expos\u00e9es au produit test\u00e9 selon des conditions reproduisant une exposition r\u00e9aliste. Le […]<\/p>","protected":false},"featured_media":1735,"template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[151],"product_tag":[113,116,112,114,115,117],"class_list":["post-1912","product","type-product","status-publish","has-post-thumbnail","product_cat-analyse","product_tag-activite","product_tag-activity","product_tag-analyse","product_tag-eau","product_tag-micro-organismes","product_tag-water","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\nTest COMET: single\u2011cell gel electrophoresis<\/title>\n<meta name=\"description\" content=\"COMET test\u202f: analyse ultra\u2011sensible de l\u2019ADN, d\u00e9tecte la g\u00e9notoxicit\u00e9 et assure la conformit\u00e9 cosm\u00e9tique Suisse\u2011UE pour les ingr\u00e9dients innovants!!!\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" 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