{"id":1922,"date":"2025-12-25T20:00:54","date_gmt":"2025-12-25T19:00:54","guid":{"rendered":"https:\/\/www.laboratoireslelegard.com\/?post_type=product&p=1922"},"modified":"2025-12-25T20:08:47","modified_gmt":"2025-12-25T19:08:47","slug":"test-tunel-adn","status":"publish","type":"product","link":"https:\/\/www.laboratoireslelegard.com\/fr_ch\/product-2\/test-tunel-adn\/","title":{"rendered":"test TUNEL: D\u00e9tecter la fragmentation de l\u2019ADN"},"content":{"rendered":"
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Test TUNEL\u202f: d\u00e9tection ultrasensible de l\u2019apoptose pour garantir la s\u00e9curit\u00e9 cellulaire des cosm\u00e9tiques<\/strong><\/p><\/div>

Le test TUNEL est une m\u00e9thode essentielle pour d\u00e9tecter la fragmentation de l\u2019ADN, un marqueur caract\u00e9ristique de la mort cellulaire programm\u00e9e, ou apoptose. Il permet de d\u00e9terminer si un ingr\u00e9dient ou un produit cosm\u00e9tique peut induire une apoptose excessive ou non d\u00e9sir\u00e9e dans des cellules expos\u00e9es, un param\u00e8tre important pour garantir la s\u00e9curit\u00e9 biologique des formulations.<\/p>

Les cellules sont d\u2019abord mises en contact avec le produit dans des conditions strictement contr\u00f4l\u00e9es, repr\u00e9sentatives de son utilisation r\u00e9elle. Les fragments d\u2019ADN g\u00e9n\u00e9r\u00e9s au cours du processus apoptotique sont ensuite marqu\u00e9s \u00e0 l\u2019aide d\u2019indicateurs fluorescents ou colorim\u00e9triques, permettant de visualiser avec pr\u00e9cision les cellules affect\u00e9es. La proportion de cellules marqu\u00e9es ainsi que l\u2019intensit\u00e9 du signal permettent d\u2019\u00e9valuer l\u2019ampleur de l\u2019effet observ\u00e9.<\/p>

Un taux \u00e9lev\u00e9 de cellules positives peut r\u00e9v\u00e9ler un risque potentiel pour la viabilit\u00e9 cellulaire, tandis qu\u2019un taux faible ou nul confirme la bonne tol\u00e9rance du produit \u00e0 ce niveau biologique. Gr\u00e2ce \u00e0 sa sensibilit\u00e9 \u00e9lev\u00e9e, le test TUNEL constitue un outil compl\u00e9mentaire aux autres analyses toxicologiques et g\u00e9n\u00e9tiques utilis\u00e9es en Suisse et en Europe pour valider l\u2019innocuit\u00e9 des ingr\u00e9dients actifs et des formulations innovantes.<\/p>

Le test TUNEL<\/strong> (Terminal deoxynucleotidyl transferase dUTP Nick End Labelling) est aujourd\u2019hui la m\u00e9thode de r\u00e9f\u00e9rence pour identifier la fragmentation de l\u2019ADN, marqueur d\u00e9finitif de la mort cellulaire programm\u00e9e (apoptose). Dans le domaine cosm\u00e9tique, il permet de v\u00e9rifier qu\u2019un ingr\u00e9dient ou une formulation ne d\u00e9clenche pas une apoptose excessive, crit\u00e8re essentiel pour la s\u00e9curit\u00e9 biologique des produits destin\u00e9s \u00e0 la peau ou aux muqueuses.<\/p><\/div>

Principe du test<\/h3><\/div>
  1. Exposition contr\u00f4l\u00e9e<\/strong> \u2013 Des cellules humaines (k\u00e9ratinocytes, fibroblastes ou cellules conjonctivales selon le produit test\u00e9) sont incub\u00e9es avec le produit \u00e0 des concentrations reproduisant les conditions d\u2019usage r\u00e9el.<\/li>
  2. Marquage des cassures d\u2019ADN<\/strong> \u2013 La nucl\u00e9otidyl\u2011transf\u00e9rase terminale (TdT) ajoute des nucl\u00e9otides marqu\u00e9s (fluorochromes\u202f: FITC, Alexa\u202f488\u202f; ou substrats colorim\u00e9triques) aux extr\u00e9mit\u00e9s 3\u2032\u2011OH des fragments d\u2019ADN g\u00e9n\u00e9r\u00e9s pendant l\u2019apoptose.<\/li>
  3. D\u00e9tection<\/strong> \u2013 Les cellules marqu\u00e9es sont visualis\u00e9es au microscope \u00e0 fluorescence ou analys\u00e9es par cytom\u00e9trie en flux. Le pourcentage de cellules positives et l\u2019intensit\u00e9 du signal quantifient l\u2019ampleur de l\u2019apoptose induite.<\/li><\/ol>

    Pourquoi le TUNEL ?<\/h3>
    • Sensibilit\u00e9 \u00e9lev\u00e9e<\/strong>\u202f: il d\u00e9tecte m\u00eame de faibles niveaux de fragmentation d\u2019ADN, souvent invisibles avec les tests de viabilit\u00e9 classiques (MTT, Trypan Blue).<\/li><\/ul><\/div>
      • \u00a0<\/li>
      • Sp\u00e9cificit\u00e9<\/strong>\u202f: la coloration ne survient que lorsque les brins d\u2019ADN sont r\u00e9ellement coup\u00e9s, \u00e9vitant les faux positifs li\u00e9s \u00e0 des dommages superficiels.<\/li>
      • Compatibilit\u00e9 avec les exigences r\u00e9glementaires<\/strong>\u202f: les autorit\u00e9s suisses (OCos\/OSAV) et europ\u00e9ennes (R\u00e8glement\u202fCE\u202f1223\/2009) exigent une \u00e9valuation compl\u00e8te de la toxicit\u00e9 cellulaire pour les ingr\u00e9dients actifs, notamment ceux d\u2019origine naturelle ou biotechnologique.<\/li>
      • Int\u00e9gration au DIP\/PIF<\/strong>\u202f: les r\u00e9sultats (pourcentage de cellules TUNEL\u2011positives, courbes dose\u2011r\u00e9ponse) sont directement report\u00e9s dans le Dossier d\u2019Information Produit (DIP) ou le Product Information File (PIF), renfor\u00e7ant le dossier de mise sur le march\u00e9.<\/li><\/ul>
        <\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

        Test TUNEL\u202f: d\u00e9tection ultrasensible de l\u2019apoptose pour garantir la s\u00e9curit\u00e9 cellulaire des cosm\u00e9tiques Le test TUNEL est une m\u00e9thode essentielle pour d\u00e9tecter la fragmentation de l\u2019ADN, un marqueur caract\u00e9ristique de la mort cellulaire programm\u00e9e, ou apoptose. Il permet de d\u00e9terminer si un ingr\u00e9dient ou un produit cosm\u00e9tique peut induire une apoptose excessive ou non d\u00e9sir\u00e9e […]<\/p>","protected":false},"featured_media":1736,"template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[151],"product_tag":[113,116,112,114,115,117],"class_list":{"0":"post-1922","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-analyse","7":"product_tag-activite","8":"product_tag-activity","9":"product_tag-analyse","10":"product_tag-eau","11":"product_tag-micro-organismes","12":"product_tag-water","14":"first","15":"instock","16":"shipping-taxable","17":"product-type-simple"},"acf":[],"yoast_head":"\nTest TUNEL: d\u00e9tecter la fragmentation de l\u2019ADN<\/title>\n<meta name=\"description\" content=\"Test TUNEL\u202f: d\u00e9tection ultrasensible de la fragmentation ADN et de l\u2019apoptose, assure la s\u00e9curit\u00e9 des cosm\u00e9tiques, conforme aux exigences 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