{"id":1922,"date":"2025-12-25T20:00:54","date_gmt":"2025-12-25T19:00:54","guid":{"rendered":"https:\/\/www.laboratoireslelegard.com\/?post_type=product&p=1922"},"modified":"2025-12-25T20:08:47","modified_gmt":"2025-12-25T19:08:47","slug":"prueba-de-adn-en-tunel","status":"publish","type":"product","link":"https:\/\/www.laboratoireslelegard.com\/es\/product\/prueba-de-adn-en-tunel\/","title":{"rendered":"Prueba TUNEL: Detectar la fragmentaci\u00f3n del ADN"},"content":{"rendered":"
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Prueba TUNEL: detecci\u00f3n ultrasensible de la apoptosis para garantizar la seguridad celular de los cosm\u00e9ticos.<\/strong><\/p><\/div>

La prueba TUNEL es un m\u00e9todo esencial para detectar la fragmentaci\u00f3n del ADN, un marcador caracter\u00edstico de la muerte celular programada o apoptosis. Permite determinar si un ingrediente o producto cosm\u00e9tico puede inducir una apoptosis excesiva o no deseada en las c\u00e9lulas expuestas, un par\u00e1metro importante para garantizar la seguridad biol\u00f3gica de las formulaciones.<\/p>

Las c\u00e9lulas se ponen primero en contacto con el producto en condiciones estrictamente controladas, representativas de su uso real. A continuaci\u00f3n, los fragmentos de ADN generados durante el proceso apopt\u00f3tico se marcan con indicadores fluorescentes o colorim\u00e9tricos, lo que permite visualizar con precisi\u00f3n las c\u00e9lulas afectadas. La proporci\u00f3n de c\u00e9lulas marcadas y la intensidad de la se\u00f1al permiten evaluar la magnitud del efecto observado.<\/p>

Un \u00edndice elevado de c\u00e9lulas positivas puede revelar un riesgo potencial para la viabilidad celular, mientras que un \u00edndice bajo o nulo confirma la buena tolerancia del producto a este nivel biol\u00f3gico. Gracias a su elevada sensibilidad, la prueba TUNEL constituye una herramienta complementaria a otros an\u00e1lisis toxicol\u00f3gicos y gen\u00e9ticos utilizados en Suiza y en Europa para validar la inocuidad de los ingredientes activos y las formulaciones innovadoras.<\/p>

El prueba TUNEL<\/strong> (Terminal deoxynucleotidyl transferase dUTP Nick End Labelling) es actualmente el m\u00e9todo de referencia para identificar la fragmentaci\u00f3n del ADN, marcador definitivo de la muerte celular programada (apoptosis). En el \u00e1mbito cosm\u00e9tico, permite verificar que un ingrediente o una formulaci\u00f3n no desencadena una apoptosis excesiva, criterio esencial para la seguridad biol\u00f3gica de los productos destinados a la piel o las mucosas.<\/p><\/div>

Principio de la prueba<\/h3><\/div>
  1. Exposici\u00f3n controlada<\/strong> \u2013 Las c\u00e9lulas humanas (queratinocitos, fibroblastos o c\u00e9lulas conjuntivales, seg\u00fan el producto sometido a prueba) se incuban con el producto en concentraciones que reproducen las condiciones de uso real.<\/li>
  2. Marcado de roturas de ADN<\/strong> \u2013 La nucleotidil transferasa terminal (TdT) a\u00f1ade nucle\u00f3tidos marcados (fluorocromos: FITC, Alexa 488; o sustratos colorim\u00e9tricos) a los extremos 3'-OH de los fragmentos de ADN generados durante la apoptosis.<\/li>
  3. Detecci\u00f3n<\/strong> \u2013 Las c\u00e9lulas marcadas se visualizan con un microscopio de fluorescencia o se analizan mediante citometr\u00eda de flujo. El porcentaje de c\u00e9lulas positivas y la intensidad de la se\u00f1al cuantifican la magnitud de la apoptosis inducida.<\/li><\/ol>

    \u00bfPor qu\u00e9 el T\u00daNEL?<\/h3>
    • Alta sensibilidad<\/strong>\u202f: detecta incluso niveles bajos de fragmentaci\u00f3n del ADN, a menudo invisibles con las pruebas de viabilidad convencionales (MTT, azul de tripano).<\/li><\/ul><\/div>
      • \u00a0<\/li>
      • Especificidad<\/strong>\u202f: la coloraci\u00f3n solo se produce cuando los fragmentos de ADN est\u00e1n realmente cortados, lo que evita los falsos positivos relacionados con da\u00f1os superficiales.<\/li>
      • Compatibilidad con los requisitos normativos<\/strong>\u202f: las autoridades suizas (OCos\/OSAV) y europeas (Reglamento CE 1223\/2009) exigen una evaluaci\u00f3n completa de la toxicidad celular para los ingredientes activos, en particular los de origen natural o biotecnol\u00f3gico.<\/li>
      • Integraci\u00f3n en el DIP\/PIF<\/strong>\u202f: los resultados (porcentaje de c\u00e9lulas TUNEL positivas, curvas dosis-respuesta) se incluyen directamente en el Dossier de Informaci\u00f3n del Producto (DIP) o en el Product Information File (PIF), lo que refuerza el expediente de comercializaci\u00f3n.<\/li><\/ul>
        <\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

        Test TUNEL\u202f: d\u00e9tection ultrasensible de l\u2019apoptose pour garantir la s\u00e9curit\u00e9 cellulaire des cosm\u00e9tiques Le test TUNEL est une m\u00e9thode essentielle pour d\u00e9tecter la fragmentation de l\u2019ADN, un marqueur caract\u00e9ristique de la mort cellulaire programm\u00e9e, ou apoptose. Il permet de d\u00e9terminer si un ingr\u00e9dient ou un produit cosm\u00e9tique peut induire une apoptose excessive ou non d\u00e9sir\u00e9e […]<\/p>","protected":false},"featured_media":1736,"template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[151],"product_tag":[113,116,112,114,115,117],"class_list":["post-1922","product","type-product","status-publish","has-post-thumbnail","product_cat-analyse","product_tag-activite","product_tag-activity","product_tag-analyse","product_tag-eau","product_tag-micro-organismes","product_tag-water","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\nTest TUNEL: d\u00e9tecter la fragmentation de l\u2019ADN<\/title>\n<meta name=\"description\" content=\"Test TUNEL\u202f: d\u00e9tection ultrasensible de la fragmentation ADN et de l\u2019apoptose, assure la s\u00e9curit\u00e9 des cosm\u00e9tiques, conforme aux exigences Suisse\u2011UE.\" \/>\n<meta name=\"robots\" content=\"index, follow, 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