{"id":1903,"date":"2025-12-25T19:34:46","date_gmt":"2025-12-25T18:34:46","guid":{"rendered":"https:\/\/www.laboratoireslelegard.com\/?post_type=product&#038;p=1903"},"modified":"2025-12-25T19:48:48","modified_gmt":"2025-12-25T18:48:48","slug":"irritacion-ocular-por-la-camara","status":"publish","type":"product","link":"https:\/\/www.laboratoireslelegard.com\/es\/product\/irritacion-ocular-por-la-camara\/","title":{"rendered":"HET-CAM: prueba de evaluaci\u00f3n de la irritaci\u00f3n ocular"},"content":{"rendered":"<div data-elementor-type=\"product-post\" data-elementor-id=\"1903\" class=\"elementor elementor-1903\" data-elementor-post-type=\"product\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-06bf5d0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"06bf5d0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6f2a6e1\" data-id=\"6f2a6e1\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7d75f8c elementor-widget elementor-widget-text-editor\" data-id=\"7d75f8c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"w-full\"><div class=\"progressive-markdown-content\"><div class=\"w-full\"><div class=\"progressive-markdown-content\"><div class=\"content-visibility-auto\"><div class=\"content-visibility-auto\"><p><strong>HET-CAM: prueba alternativa sin animales para evaluar la irritaci\u00f3n ocular.<\/strong><\/p><\/div><div class=\"content-visibility-auto\"><p>El <strong>HET-CAM<\/strong> (Prueba del huevo de gallina \u2013 Membrana corioalantoidea) es actualmente el m\u00e9todo de referencia para evaluar el potencial irritante de un producto cosm\u00e9tico en los ojos sin recurrir a la experimentaci\u00f3n con animales. De conformidad con los requisitos europeos (Reglamento CE 1223\/2009) y suizos (OCos\/OSAV), esta prueba utiliza la membrana corioalantoidea de un huevo de gallina incubado: una membrana ultrafina, muy vascularizada y extremadamente sensible a los agentes qu\u00edmicos irritantes.<\/p><\/div><div class=\"content-visibility-auto\"><h3>Principio de la prueba<\/h3><\/div><div class=\"content-visibility-auto\"><ol><li><strong>Incubaci\u00f3n del huevo<\/strong> \u2013 El huevo se incuba durante 9 a 10 d\u00edas a 37 \u00b0C, momento en el que la membrana corioalantoidea est\u00e1 completamente desarrollada.<\/li><li><strong>Aplicaci\u00f3n del producto<\/strong> \u2013 Se deposita una peque\u00f1a gota (10 \u00b5L) del producto cosm\u00e9tico que se va a analizar directamente sobre la membrana.<\/li><li><strong>Marcado de lesiones<\/strong> \u2013 Se a\u00f1aden colorantes espec\u00edficos (generalmente rojo de fenolsulfonato) para resaltar las alteraciones vasculares: enrojecimiento, microcoagulaciones, hemorragias o rotura de vasos.<\/li><li><strong>Observaci\u00f3n y puntuaci\u00f3n<\/strong> \u2013 Se observa la membrana durante 5 minutos y, a continuaci\u00f3n, a los 30 minutos. Se anota la extensi\u00f3n y la rapidez de los cambios vasculares seg\u00fan una tabla de puntuaci\u00f3n estandarizada (0 = sin irritaci\u00f3n, 5 = irritaci\u00f3n grave).<\/li><\/ol><h3>Ventajas del HET-CAM<\/h3><ul><li><strong>Ausencia de animales maduros<\/strong>\u202f: el m\u00e9todo utiliza un embri\u00f3n, lo que lo clasifica entre las t\u00e9cnicas 3R (Reemplazo).<\/li><li><strong>Alta sensibilidad<\/strong>\u202f: incluso los irritantes leves provocan respuestas vasculares visibles, lo que permite una clasificaci\u00f3n detallada seg\u00fan el sistema GHS.<\/li><li><strong>R\u00e1pidamente ejecutable<\/strong>\u202f: el protocolo completo se lleva a cabo en menos de una hora, lo que resulta ideal para las fases de desarrollo en las que los plazos son cr\u00edticos.<\/li><li><strong>Coste reducido<\/strong>\u202f: no se necesitan equipos costosos ni mantenimiento de animales, lo que hace que la prueba resulte econ\u00f3micamente atractiva para los laboratorios de I+D.<\/li><\/ul><h3>L\u00edmites que hay que conocer<\/h3><ul><li><strong>No se mide la permeabilidad corneal.<\/strong>\u202f: a diferencia del BCOP o de los modelos de c\u00f3rnea reconstruida, el HET-CAM no proporciona informaci\u00f3n directa sobre la penetraci\u00f3n del producto en la c\u00f3rnea.<\/li><li><strong>Interpretaci\u00f3n semicuantitativa<\/strong>\u202f: la puntuaci\u00f3n se basa en la observaci\u00f3n visual, lo que puede introducir una variabilidad entre operadores si los criterios no est\u00e1n estrictamente armonizados.<\/li><li><strong>Aplicabilidad a formulaciones muy lipof\u00edlicas<\/strong>\u202f: los aceites espesos pueden no distribuirse uniformemente sobre la membrana, lo que a veces requiere la adici\u00f3n de un disolvente compatible.<\/li><\/ul><h3>Integraci\u00f3n en el expediente reglamentario<\/h3><p>Los resultados del HET-CAM se incorporan al <strong>DIP\/PIF<\/strong> (Expediente de informaci\u00f3n del producto \/ Product Information File) y sirven para justificar la clasificaci\u00f3n de irritaci\u00f3n ocular del producto. Son especialmente relevantes para los <strong>cremas para el contorno de ojos, m\u00e1scaras de pesta\u00f1as, desmaquillantes oculares<\/strong> y cualquier otro producto que pueda entrar en contacto con la zona ocular.<\/p><p>Al combinar el HET-CAM con otras pruebas in vitro (BCOP, RhCE) para cubrir la permeabilidad corneal, los fabricantes obtienen un expediente completo que cumple con los requisitos suizos, europeos y brit\u00e1nicos. Este enfoque hol\u00edstico refuerza la credibilidad cient\u00edfica del producto, acelera la obtenci\u00f3n del n\u00famero de notificaci\u00f3n y tranquiliza a los consumidores en cuanto a la seguridad de los cuidados cerca de los ojos.<\/p><\/div><br \/><hr \/><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>HET-CAM: prueba alternativa sin animales para evaluar la irritaci\u00f3n ocular. El HET-CAM (Hen\u2019s Egg Test \u2013 Membrana corioalantoidea) es actualmente el m\u00e9todo de referencia a la hora de evaluar el potencial irritante de un producto cosm\u00e9tico para los ojos sin recurrir a la experimentaci\u00f3n con animales. De conformidad con los requisitos europeos (Reglamento (CE) n.\u00ba 1223\/2009) y suizos (OCos\/OSAV), esta prueba utiliza la membrana corioalantoidea [\u2026]<\/p>","protected":false},"featured_media":1738,"template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[151],"product_tag":[113,116,112,114,115,117],"class_list":["post-1903","product","type-product","status-publish","has-post-thumbnail","product_cat-analyse","product_tag-activite","product_tag-activity","product_tag-analyse","product_tag-eau","product_tag-micro-organismes","product_tag-water","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>HET\u2011CAM\u202f: test d\u2019\u00e9valuation de l\u2019irritation oculaire<\/title>\n<meta name=\"description\" content=\"HET-CAM : test alternatif sans animaux pour \u00e9valuer l\u2019irritation oculaire, fiable 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