{"id":1903,"date":"2025-12-25T19:34:46","date_gmt":"2025-12-25T18:34:46","guid":{"rendered":"https:\/\/www.laboratoireslelegard.com\/?post_type=product&p=1903"},"modified":"2025-12-25T19:48:48","modified_gmt":"2025-12-25T18:48:48","slug":"eye-irritation","status":"publish","type":"product","link":"https:\/\/www.laboratoireslelegard.com\/en\/product\/eye-irritation\/","title":{"rendered":"HET-CAM: eye irritation assessment test"},"content":{"rendered":"
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t

HET-CAM: alternative non-animal test for assessing eye irritation<\/strong><\/p><\/div>

The HET-CAM<\/strong> (Hen's Egg Test \u2013 Chorioallantoic Membrane) is now the gold standard method for assessing the irritant potential of a cosmetic product on the eyes without resorting to animal testing. In accordance with European (Regulation EC 1223\/2009) and Swiss (OCos\/OSAV) requirements, this test uses the chorioallantoic membrane of an incubated chicken egg: an ultra-thin membrane that is highly vascularized and extremely sensitive to chemical irritants.<\/p><\/div>

Test principle<\/h3><\/div>
  1. Incubation of the egg<\/strong> \u2013 The egg is incubated for 9 to 10 days at 37\u00b0C, at which point the chorioallantoic membrane is fully developed.<\/li>
  2. Product application<\/strong> \u2013 A small drop (10 \u00b5L) of the cosmetic product to be tested is placed directly on the membrane.<\/li>
  3. Marking lesions<\/strong> \u2013 Specific dyes (usually phenol sulfonate red) are added to highlight vascular changes: redness, microcoagulations, hemorrhages, or vessel rupture.<\/li>
  4. Observation and scoring<\/strong> \u2013 The membrane is observed for 5 minutes, then again after 30 minutes. The extent and speed of vascular changes are noted according to a standardized scoring table (0 = no irritation, 5 = severe irritation).<\/li><\/ol>

    Benefits of HET-CAM<\/h3>
    • Absence of mature animals<\/strong>\u202f: the method uses an embryo, which classifies it as a 3R (Replacement) technique.<\/li>
    • High sensitivity<\/strong>\u202f: even mild irritants cause visible vascular responses, allowing for detailed classification according to the GHS system.<\/li>
    • Quickly executable<\/strong>\u202f: The entire protocol takes less than an hour, ideal for development phases where time is of the essence.<\/li>
    • Reduced cost<\/strong>\u202f: no need for expensive equipment or animal maintenance, making the test economically attractive for R&D laboratories.<\/li><\/ul>

      Limitations to be aware of<\/h3>
      • No corneal permeability measurement<\/strong>\u202fUnlike BCOP or reconstituted corneal models, HET-CAM does not provide direct information on the penetration of the product into the cornea.<\/li>
      • Semi-quantitative interpretation<\/strong>\u202f: scoring is based on visual observation, which can introduce inter-operator variability if the criteria are not strictly harmonized.<\/li>
      • Applicability to highly lipophilic formulations<\/strong>\u202f: thick oils may not spread evenly over the membrane, sometimes requiring the addition of a compatible solvent.<\/li><\/ul>

        Inclusion in the regulatory file<\/h3>

        The HET-CAM results are incorporated into the DIP\/PIF<\/strong> (Product Information File) and are used to justify the product's eye irritation classification. They are particularly relevant for eye creams, mascaras, eye makeup removers<\/strong> and any other product that may come into contact with the eye area.<\/p>

        By combining HET-CAM with other in vitro tests (BCOP, RhCE) to cover corneal permeability, manufacturers obtain a complete dossier that complies with Swiss, European, and UK requirements. This holistic approach reinforces the scientific credibility of the product, speeds up the process of obtaining a notification number, and reassures consumers about the safety of their eye care products.<\/p><\/div>

        <\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

        HET-CAM: An Alternative, Non-Animal Test for Assessing Eye Irritation HET-CAM (Hen\u2019s Egg Test \u2013 Chorioallantoic Membrane) is currently the gold standard for assessing the eye-irritating potential of a cosmetic product without resorting to animal testing. In accordance with European (Regulation (EC) No. 1223\/2009) and Swiss (OCos\/OSAV) requirements, this test utilizes the chorioallantoic membrane [\u2026]<\/p>","protected":false},"featured_media":1738,"template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[151],"product_tag":[113,116,112,114,115,117],"class_list":["post-1903","product","type-product","status-publish","has-post-thumbnail","product_cat-analyse","product_tag-activite","product_tag-activity","product_tag-analyse","product_tag-eau","product_tag-micro-organismes","product_tag-water","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\nHET\u2011CAM\u202f: test d\u2019\u00e9valuation de l\u2019irritation oculaire<\/title>\n<meta name=\"description\" content=\"HET-CAM : test alternatif sans animaux pour \u00e9valuer l\u2019irritation oculaire, fiable pour cosm\u00e9tiques, conforme aux exigences UE\u2011Suisse et la r\u00e9glementation!\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.laboratoireslelegard.com\/en\/product\/eye-irritation\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"HET\u2011CAM\u202f: test d\u2019\u00e9valuation de l\u2019irritation oculaire\" \/>\n<meta property=\"og:description\" content=\"HET-CAM : test alternatif sans animaux pour \u00e9valuer l\u2019irritation oculaire, fiable pour cosm\u00e9tiques, conforme aux exigences UE\u2011Suisse et la r\u00e9glementation!\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.laboratoireslelegard.com\/en\/product\/eye-irritation\/\" \/>\n<meta property=\"og:site_name\" content=\"Laboratoires Lel\u00e9gard\" \/>\n<meta property=\"article:modified_time\" content=\"2025-12-25T18:48:48+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.laboratoireslelegard.com\/wp-content\/uploads\/2023\/04\/Het-Cam.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1024\" \/>\n\t<meta property=\"og:image:height\" content=\"575\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/\",\"url\":\"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/\",\"name\":\"HET\u2011CAM\u202f: test d\u2019\u00e9valuation de l\u2019irritation oculaire\",\"isPartOf\":{\"@id\":\"https:\/\/www.laboratoireslelegard.com\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.laboratoireslelegard.com\/wp-content\/uploads\/2023\/04\/Het-Cam.jpg\",\"datePublished\":\"2025-12-25T18:34:46+00:00\",\"dateModified\":\"2025-12-25T18:48:48+00:00\",\"description\":\"HET-CAM : test alternatif sans animaux pour \u00e9valuer l\u2019irritation oculaire, fiable pour cosm\u00e9tiques, conforme aux exigences UE\u2011Suisse et la r\u00e9glementation!\",\"breadcrumb\":{\"@id\":\"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/#primaryimage\",\"url\":\"https:\/\/www.laboratoireslelegard.com\/wp-content\/uploads\/2023\/04\/Het-Cam.jpg\",\"contentUrl\":\"https:\/\/www.laboratoireslelegard.com\/wp-content\/uploads\/2023\/04\/Het-Cam.jpg\",\"width\":1024,\"height\":575,\"caption\":\"HET\u2011CAM\u202f: test d\u2019\u00e9valuation de l\u2019irritation oculaire\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\/\/www.laboratoireslelegard.com\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Boutique\",\"item\":\"https:\/\/www.laboratoireslelegard.com\/prestations-formations\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"HET\u2011CAM\u202f: test d\u2019\u00e9valuation de l\u2019irritation oculaire\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.laboratoireslelegard.com\/#website\",\"url\":\"https:\/\/www.laboratoireslelegard.com\/\",\"name\":\"Laboratoires Lel\u00e9gard\",\"description\":\"SERVICE DE REGLEMENTATION ET TOXICOLOGIE\",\"publisher\":{\"@id\":\"https:\/\/www.laboratoireslelegard.com\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.laboratoireslelegard.com\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.laboratoireslelegard.com\/#organization\",\"name\":\"Laboratoires Lel\u00e9gard\",\"url\":\"https:\/\/www.laboratoireslelegard.com\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.laboratoireslelegard.com\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.laboratoireslelegard.com\/wp-content\/uploads\/2022\/10\/labolelegard.svg\",\"contentUrl\":\"https:\/\/www.laboratoireslelegard.com\/wp-content\/uploads\/2022\/10\/labolelegard.svg\",\"width\":515,\"height\":116,\"caption\":\"Laboratoires Lel\u00e9gard\"},\"image\":{\"@id\":\"https:\/\/www.laboratoireslelegard.com\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"HET-CAM: eye irritation assessment test","description":"HET-CAM: an alternative, animal-free test for assessing eye irritation, reliable for cosmetics, compliant with EU-Swiss requirements and regulations!","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.laboratoireslelegard.com\/en\/product\/eye-irritation\/","og_locale":"en_US","og_type":"article","og_title":"HET\u2011CAM\u202f: test d\u2019\u00e9valuation de l\u2019irritation oculaire","og_description":"HET-CAM : test alternatif sans animaux pour \u00e9valuer l\u2019irritation oculaire, fiable pour cosm\u00e9tiques, conforme aux exigences UE\u2011Suisse et la r\u00e9glementation!","og_url":"https:\/\/www.laboratoireslelegard.com\/en\/product\/eye-irritation\/","og_site_name":"Laboratoires Lel\u00e9gard","article_modified_time":"2025-12-25T18:48:48+00:00","og_image":[{"width":1024,"height":575,"url":"https:\/\/www.laboratoireslelegard.com\/wp-content\/uploads\/2023\/04\/Het-Cam.jpg","type":"image\/jpeg"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/","url":"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/","name":"HET-CAM: eye irritation assessment test","isPartOf":{"@id":"https:\/\/www.laboratoireslelegard.com\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/#primaryimage"},"image":{"@id":"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/#primaryimage"},"thumbnailUrl":"https:\/\/www.laboratoireslelegard.com\/wp-content\/uploads\/2023\/04\/Het-Cam.jpg","datePublished":"2025-12-25T18:34:46+00:00","dateModified":"2025-12-25T18:48:48+00:00","description":"HET-CAM: an alternative, animal-free test for assessing eye irritation, reliable for cosmetics, compliant with EU-Swiss requirements and regulations!","breadcrumb":{"@id":"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/#primaryimage","url":"https:\/\/www.laboratoireslelegard.com\/wp-content\/uploads\/2023\/04\/Het-Cam.jpg","contentUrl":"https:\/\/www.laboratoireslelegard.com\/wp-content\/uploads\/2023\/04\/Het-Cam.jpg","width":1024,"height":575,"caption":"HET\u2011CAM\u202f: test d\u2019\u00e9valuation de l\u2019irritation oculaire"},{"@type":"BreadcrumbList","@id":"https:\/\/www.laboratoireslelegard.com\/produit\/het-cam-irritation-occulaire\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/www.laboratoireslelegard.com\/"},{"@type":"ListItem","position":2,"name":"Boutique","item":"https:\/\/www.laboratoireslelegard.com\/prestations-formations\/"},{"@type":"ListItem","position":3,"name":"HET\u2011CAM\u202f: test d\u2019\u00e9valuation de l\u2019irritation oculaire"}]},{"@type":"WebSite","@id":"https:\/\/www.laboratoireslelegard.com\/#website","url":"https:\/\/www.laboratoireslelegard.com\/","name":"Lel\u00e9gard Laboratories","description":"REGULATORY AND TOXICOLOGY DEPARTMENT","publisher":{"@id":"https:\/\/www.laboratoireslelegard.com\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.laboratoireslelegard.com\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.laboratoireslelegard.com\/#organization","name":"Lel\u00e9gard Laboratories","url":"https:\/\/www.laboratoireslelegard.com\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.laboratoireslelegard.com\/#\/schema\/logo\/image\/","url":"https:\/\/www.laboratoireslelegard.com\/wp-content\/uploads\/2022\/10\/labolelegard.svg","contentUrl":"https:\/\/www.laboratoireslelegard.com\/wp-content\/uploads\/2022\/10\/labolelegard.svg","width":515,"height":116,"caption":"Laboratoires Lel\u00e9gard"},"image":{"@id":"https:\/\/www.laboratoireslelegard.com\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/www.laboratoireslelegard.com\/en\/wp-json\/wp\/v2\/product\/1903","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.laboratoireslelegard.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.laboratoireslelegard.com\/en\/wp-json\/wp\/v2\/types\/product"}],"version-history":[{"count":8,"href":"https:\/\/www.laboratoireslelegard.com\/en\/wp-json\/wp\/v2\/product\/1903\/revisions"}],"predecessor-version":[{"id":1911,"href":"https:\/\/www.laboratoireslelegard.com\/en\/wp-json\/wp\/v2\/product\/1903\/revisions\/1911"}],"wp:attachment":[{"href":"https:\/\/www.laboratoireslelegard.com\/en\/wp-json\/wp\/v2\/media?parent=1903"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.laboratoireslelegard.com\/en\/wp-json\/wp\/v2\/product_brand?post=1903"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.laboratoireslelegard.com\/en\/wp-json\/wp\/v2\/product_cat?post=1903"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.laboratoireslelegard.com\/en\/wp-json\/wp\/v2\/product_tag?post=1903"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}